Application
Biotinylated D-lysine (BDL) can be used to conjugate a biotin handle into bacterial peptidoglycan (PG) for various applications. For example, Suzanne Walker and coworkers reported that penicillin-binding protein PBP4 of Staphylococcus aureus could use BDL to biotinylate Lipid I and Lipid II, facilitating assays to measure infamously low-abundance pools of cellular lipid-linked PG precursors. BDL provides a tool for studying other aspects of PG assembly including antibacterial development efforts.
Other Notes
Detection of Lipid-Linked Peptidoglycan Precursors by Exploiting an Unexpected Transpeptidase Reaction
Packaging
10 mg in glass bottle
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